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Multi-Omic Studies with Sequential At Sequential, our mission has always been clear: to understand the impact of the microbiome on humans and how humans impact the microbiome in order to characterize human health fully. Our Multi-Omic Studies brings us back to the core of our mission by offering an unparalleled approach to skin health research. By combining human and microbial analysis, Sequential is setting a new standard in clinical testing. This comprehensive methodology enables us to unlock deeper insights into the complex interplay between the microbiome and human biology, offering more tailored and effective product assessments for our partners in personal care and the pharmaceutical industries. *Hover over the diagram to unlock each area that we explore! Identify key genetic markers linked to health, aging, and disease susceptibility Predict responsiveness to product SNP Detection & Risk Assessment Gene Expression Profile Uncover how genes are activated in response to lifestyle, environment, and treatments The effect of product on skin-related gene expression Protein Profiling Analyse protein biomarkers to track biological changes and optimise product efficacy Measure skin protein production after a product treatment Lipid Profiling Investigate lipid composition for insights into skin barrier function and metabolic health Assess how a product restores lipid homeostasis qPCR Sequencing Investigate the absolute quantification of key microbes in any sample Assess how a product impacts the most significant microbes in a sample Metatranscriptomics Uncover how genes are activated in response to lifestyle, environment, & treatments. The effect of product on skin-related microbial gene expression ITS Sequencing Investigate all present fungi in any given sample Understand how a product impacts the skin's fungal community Shotgun Metagenomics Investigate all present microorganisms in any given sample Understand how a product impacts the skin's bacteria, fungal and viral communities 16S Sequencing Investigate all present bacteria in any given sample Understand how a product impacts the skin's bacterial community Metabolomics Map metabolic pathways to assess skin and body health, aging, and personalized wellness. Metabolic shift pre- and post-product application Start Your Multi-Omic Study Today! Why Multi-Omic Testing? Understanding how products impact both the human body and its microbiome is essential for creating truly effective solutions. Our multi-omic approach provides: Deeper Insights – See how products influence human health at a biological level Clinically Validated Science – Data-backed results to support claims and innovation Personalized Health & Wellness – Create tailored products that optimize skin and body health Scalable & Flexible Testing – In-lab or remote studies with diverse sample collection options Microbiome's Impact on Human Health Revolutionising Eczema Care: The Promise of Live Biotherapeutic Products Eczema, or atopic dermatitis (AD), is a chronic inflammatory skin condition marked by skin barrier dysfunction and immune dysregulation.... A Product of Our Environment: What Does the Exposome Mean for Skincare? The ‘exposome’ refers to the complex interplay of environmental exposures that influence the skin over a lifetime, including factors such... Preservatives: Essential for Products, But Are They Disrupting Your Skin? Introduction: Cosmetic Preservatives Cosmetics are products designed to enhance or alter the appearance of the face, body, or hair, with... Your Muti-Omics Questions Answered What is Sequential's testing platform? Sequential has developed the gold standard test for microbiome-friendly products, in vivo (in, or on, humans). Finally, we can give some certainty about if a product is truly affecting the microbiome. We offer a complete end-to-end solution to support microbiome-friendly claims. From consultancy and study design to our proprietary microbiome testing kits. We analyse, interpret and report our findings to meet your needs. Why is it necessary to test the microbiome in vivo? At present, there are no regulations for microbiome-related formulas that brands and formulators can follow, however, it has been universally acknowledged that the in vivo method of conducting clinical studies is becoming critical and paramount to getting marketing claims through. When regulations are introduced, which may be imminent, the in vitro system will find itself lacking, resulting in limited claims and certifications that do not hold their value. This is why, we at Sequential strive to offer an in vivo approach, knowing full well that we want our client's claims to be significantly backed by scientific and quantifiable data. What type of sequencing technology does Sequential use for analysis? We offer four types of sequencing techniques including qPCR with our Smart Probes™, 16S, ITS and Shotgun Metagenomics. Using next-generation sequencing of the collection of microorganisms found on the body, during product usage, Sequential investigates the microbial diversity, and particular microorganisms we know are important and play a role in a healthy microbiome. Does Sequential offer claims certification for tested products? We provide our clients with a certification to claim “Maintains the Microbiome” subject to in vivo testing results which can be used in communication efforts. Once your product is tested with our qPCR Smart Probes™ and has shown favourable results in supporting the microbiome, we can certify your product with our Maintains the Microbiome certification seal. We have ensured that our seal and certification are backed by quantifiable data and scientifically significant markers. The aim is to ensure our clients feel confident in making their claims and can communicate the true benefit of their microbiome formulations.

Sequential is proud to announce that is has been dubbed the most significant testing method in the industry for microbiome testing. Allē Award: Sequential Wins Prestigious “Most Significant” Testing Method After being listed as a finalist as best ‘Claims testing methods & tools’ alongside Evonik, and XCellR8 - Sequential brings home top prize in C&T’s Allē Awards, 2022. The personal care and cosmetics industry in the US is valued at $190B. In the context of the skin microbiome, the industry is still a relatively nascent field. However, its projected growth is significant, estimated to grow at a compounded annual growth of 29.2% between 2022-2028. Owed to the impact of products on the skin microbiome, consumer awareness, and understanding that our microbiome is intimately linked to our health. Sequential is the B2B microbiome testing arm for Sequential Skin Ltd, which has developed the world’s first end-to-end platform for companies to evaluate their products on the microbiome in vivo. They specialize in skin, scalp and intimate area microbiome. "Of all the 'microbiome friendly' methodologies working to address both consumer and industry concerns about the impact of cosmetics on the microbiome, this is, in my opinion, the most quantitative and promising of the lot." – C&T's Allē Award judges, 2022. The company has developed a proprietary non-invasive method for collecting skin samples to analyze the skin microbiome using next-generation sequencing (NGS), with species and strain identification, in longitudinal (and clinical) studies. Services include data interpretation and the results are reported in a comprehensive, yet understandable format, ready for formulators and research scientists to incorporate into their product development pipeline. “The Allē Award gives us strong confidence in the testing platform and capabilities we’ve built in Sequential. Being the first to develop an in vivo microbiome test for the industry, we’re proud to see our hard work paying off” — Petronille Houdart, DPharm, skincare director at Sequential. About Sequential Sequential is part of the Sequential Skin group – with a US lab in New York City, alongside a lab in London and a lab in Singapore. Sequential team has over 20 years of combined expertise in genetics, epigenetics, and microbiome research. Sequential has validated its AI-driven testing platform with over 30 companies, analyzing over 12,000 skin microbiome samples. They specialise in skin, scalp and vulva/vaginal microbiome samples. They are supported by Enterprise SG, A*STAR, Genome Institute of Singapore, IndieBio New York, SOSV, Metaplanet Holdings, Scrum Ventures, Genedant VC, Ben Holmes (ex. General Partner at Index Ventures), Innovate UK, and are a resident company of Johnson & Johnson Innovation – JLABS.

Download your Personal Skin Health Tracker app by Sequential Skin to receive your microbiome test results and unlock personalized skincare tips. Get it now! The skin health tracker Benefit of your personal Skin Health Tracker Download your personal Skin Health Tracker app to receive your microbiome test results and unlock personalized skincare tips. Results Skin Profile Receive your comprehensive skin microbiome sample results. Results Accessibility Access to your Skin Profile results in the palm of your hand. Results Unlock Our Expertise Learn skincare tips to help you with your own perceived skin traits. Results Discover Learn more about the skin microbiome through our Discover articles. What is Sequential's testing platform? Sequential has developed the gold standard test for microbiome-friendly products, in vivo (in, or on, humans). Finally, we can give some certainty about if a product is truly affecting the microbiome. We offer a complete end-to-end solution to support microbiome-friendly claims. From consultancy and study design to our proprietary microbiome testing kits. We analyse, interpret and report our findings to meet your needs. Why is it necessary to test the microbiome in vivo? At present, there are no regulations for microbiome-related formulas that brands and formulators can follow, however, it has been universally acknowledged that the in vivo method of conducting clinical studies is becoming critical and paramount to getting marketing claims through. When regulations are introduced, which may be imminent, the in vitro system will find itself lacking, resulting in limited claims and certifications that do not hold their value. This is why, we at Sequential strive to offer an in vivo approach, knowing full well that we want our client's claims to be significantly backed by scientific and quantifiable data. What type of sequencing technology does Sequential use for analysis? We offer four types of sequencing techniques including qPCR with our Smart Probes™, 16S, ITS and Shotgun Metagenomics. Using next-generation sequencing of the collection of microorganisms found on the body, during product usage, Sequential investigates the microbial diversity, and particular microorganisms we know are important and play a role in a healthy microbiome. Does Sequential offer claims certification for tested products? We provide our clients with a certification to claim “Maintains the Microbiome” subject to in vivo testing results which can be used in communication efforts. Once your product is tested with our qPCR Smart Probes™ and has shown favourable results in supporting the microbiome, we can certify your product with our Maintains the Microbiome certification seal. We have ensured that our seal and certification are backed by quantifiable data and scientifically significant markers. The aim is to ensure our clients feel confident in making their claims and can communicate the true benefit of their microbiome formulations. Unearth the secrets of your microbiome

The ingredient list for our Biome Mask formulation with each of their applications from giving deep hydration to protecting from pollutants with antioxidant benefits. Category Ingredient What is it? Benefit Melanin Targeters Arbutin Arbutin is an antioxidant that is extracted from different berries. Arbutin helps in ensuring that skin is brightened and pigmentation is reduced in the skin. It can also fade scars. Hydrators Squalane Squalane is a hydrogenated version of squalene. It is an emollient and behaves similar to our own skin sebum. Prevents moisture loss, can neutralize UV damage . Postbiotics Lactobacillus ferment Lactobacillus ferment is a non-living probiotic ingredient made by fermentation of the microorganism Lactobacillus. Thanks to its soothing effect on the skin, it helps to strengthen the skin's defense and maintains the health of the skin microbiome. Allantoin Allantoin is an extract from comfrey plant. Allantoin protects and soothes the skin. Trehalose Trehalose is a disaccharide with hydrating properties for the skin. It’s a plant sugar. Trehalose is used as a prebiotic and is effective to help maintain healthy skin. Green Tea Extract Extract from the leaves of green tea. Tea Extracts is an antioxidant ingredient that aids soothing the skin and can also address skin discoloration and early signs of aging. Colloidal Oatmeal Colloidal Oatmeal is cocktail of oats that has been refined into powder and acts as a skin protectant. Colloidal Oatmeal aids in locking in moisture in the skin barrier and also has soothing properties. Skin Soothing Multi-Ceramide [Ceramide NP; Phytosphingosine] Ceramides are lipids or fats that exist in skin cells and make up to 40% of your outer skin layer. Ceramides are crucial in aiding your skin to retain moisture and prevent unwanted bacteria from entering your body. Skin Repairing Peptide Complex [copper tripeptide-1, Acetyl heaxapeptide-8, Palmitoyl Pentapepdide-4, Palmitoyl tripeptide-1, Palmitoyl tetrapepide-7, tripeptide-1, tripeptide-2, tripeptide-2, tripeptide-3, acetyl Octapeptide 3] Peptides are amino acid chains that aid in the forming of certain types of protein in the skin such as elastin and collagen. Peptides can help in increasing skin elasticity and reducing signs of aging, allowing skin to appear firmer. Skin Shields Vitamin E [Tocopherol] Tocopherol is an excellent example of a powerful antioxidant that is oil-soluble, meaning it can easily dissolve into the skin. Tocopherol helps in combating free-radical damage (such as pollution), and can also be an effective hydrator. Retinyl Palmitate Retinyl Palmitate is the most abundantly used form of retinol (Vitamin A). Retinyl Palmitate has anti-aging properties as well as anti-oxidant properties. Centella Asiatica Centella Asiatica is a medicinal herb that is known to treat inflammation and has antioxidant properties. Centella Asiatica is used as an active ingredient in skincare products for anti-inflammation, anti-oxidant and soothes compromised skin. Sorbitol Sorbitol is a sugar with hydrating properties that can nourish the probiotics that live on the skin allowing the skin microbiome to be balanced. Sorbitol breakdown by microorganisms on the skin creates beta-glucan, a skin calming antioxidant. Skin Barrier Protectors (Hydrators) Hyaluronic Acid Hyaluronic Acid is a humectant, meaning that it attracts water from the air or within the skin. Hyaluaronic Acid is one of the most common known ingredients for increasing overall moisture in skin. Vitamin C [Ascorbic Acid] Ascorbic Acid is an antioxidant and the most stable Vitamin C form. Ascorbic Acid aids in brightening the skin and aids in the production of collagen to prevent premature aging. Prebiotics Fructooligosaccharides Fructooligosaccharides is a prebiotic sugar used as food by the skin microbiota. It’s also a good source of antioxidants. It helps to keep the skin clean, hydrated, and well nutrified. It helps to maintain a healthy microbiome for your skin. Niacinamide Niacinamide is a vitamin that is water-soluble and penetrate into the skin with ease. Niacinamide aids in reducing the visibility of pores and removes dullness and restores the skin. Licorice Root Licorice Root is a plant based ingredients that can be cultivated in Asia, Europe, and in the Middle East. Licorice Root is a brightening agent in skincare products and can repair the skin. Lactic Acid Lactic Acid is a metabolic by-product released by the fermentation of microorganisms like lactobacillus. Lactic acid strengthens the skin barrier and has a positive impact on the skin microbiome balance. It also helps in reducing uneven tone and brightens skin tone.

Ensure your microbiome-centric scalp product excels. Obtain expert certification to amplify its benefits in your messaging. Contact us today. Scalp Microbiome Testing As the scalp care industry grows, consumers demand transparency from the brands formulating their products. It has become essential for formulators to create products they are willing to test to present scientifically backed data-driven evidence of their products' true effects. Sequential offers microbiome testing for your scalp care formulations, ranging from scalp serums, shampoos, conditioners, oils, etc. We are dedicated to understanding how your product interacts with the scalp and its microbes. Depending on how in-depth you want to go, we offer qPCR, 16S, ITS, and Shotgun Metagenomics. Download Case Study! Personalized Approach to Testing Unlike other methodologies present within the industry, Sequential's approach ensures that your product's data and analysis will stand the test of industry regulations when they are introduced. You can tailor your study entirely to your unique requirements. Test Products in a Real-Life Context The microbiome comprises a complex ecosystem of microorganisms that live together in a delicate balance, which is why it's best to test directly upon it directly. To fully understand the impact a product is having on the microbiome, in vivo is the only way. Collect Longitudinal Data With in vivo testing we can design your study around the extended use of a product over multiple time points. This allows us to review how a product is performing before and after usage, but also take into account its gradual impact on the microbiome. Measure Against a Control Group Measure a product against a control group that might have a different percentage of your active ingredient within its formulation or no active at all. This will allow for deeper insights into the impact of a formulation on microbial balance and diversity. 4 Sequencing Reports To Pick From Depending on your development stage and what you are interested in studying we offer qPCR, 16S, ITS, and Shotgun Metagenomics. With our qPCR Smart Probes™ we can go down to the strain level in our analysis. Personalize Your Microbiome Study! Unlike hair, the scalp is formed of a living community of microorganisms such as bacterial and fungal players that can influence the balance of the scalp microbiome, even the subtlest of imbalances can lead to issues such as dandruff, itching, and irritation, resulting in unwanted flaking. Smart Probe s ™ Our dedicated team of scientists have developed a method of evaluating microbes through our Smart Probes ™ . These refer to a panel of 20 key microbes we have specifically identified as having the most impact on scalp health. Over and above the taxonomic characterisation that 16S offers, which gives us a snapshot of all the genus present within a collected sample (Cutibacterium , Staphylococcus , etc.) our targeted approach takes it a step further, opening the lens to the species (C. acnes ), sub-species (C. acnes defendens ) and even strains within them. This is a crucial distinction as not all strains of a species behave similarly. We find that within these species there are strains associated with inflammation and strains that are commensal, and beneficial. Gold Standard Certification Sequential has developed the gold standard test for products designed to target the microbiome, in vivo (in, or on, humans). Finally, we can give some certainty about if a product is truly affecting the microbiome. Using next-generation sequencing of the collection of micro-organisms found on the body, before and after product usage, Sequential investigates the microbial balance and diversity, and particular micro-organisms we know are important and play a role in a healthy microbiome. We give you an in vivo certification that your product maintains the microbiome. And it’s not exclusive to skincare! We do this for haircare products, oral products, and vulva/vaginal microbiomes. Personalize Your Microbiome Study! Supplement Your Microbiome Study Recruitment Services Let us take care of the entire candidate recruitment process for you! View More Biophysical Assessments Increase your data on the use of your product by evaluating additional biophysical factors. View More Formulation Support Seek consultation advice for your formulation if you are re-formulating or developing a new product. View More FAQ What is Sequential's testing platform? Sequential has developed the gold standard test for microbiome-friendly products, in vivo (in, or on, humans). Finally, we can give some certainty about if a product is truly affecting the microbiome. We offer a complete end-to-end solution to support microbiome-friendly claims. From consultancy and study design to our proprietary microbiome testing kits. We analyse, interpret and report our findings to meet your needs. Why is it necessary to test the microbiome in vivo? At present, there are no regulations for microbiome-related formulas that brands and formulators can follow, however, it has been universally acknowledged that the in vivo method of conducting clinical studies is becoming critical and paramount to getting marketing claims through. When regulations are introduced, which may be imminent, the in vitro system will find itself lacking, resulting in limited claims and certifications that do not hold their value. This is why, we at Sequential strive to offer an in vivo approach, knowing full well that we want our client's claims to be significantly backed by scientific and quantifiable data. What type of sequencing technology does Sequential use for analysis? We offer four types of sequencing techniques including qPCR with our Smart Probes™, 16S, ITS and Shotgun Metagenomics. Using next-generation sequencing of the collection of microorganisms found on the body, during product usage, Sequential investigates the microbial diversity, and particular microorganisms we know are important and play a role in a healthy microbiome. Does Sequential offer claims certification for tested products? We provide our clients with a certification to claim “Maintains the Microbiome” subject to in vivo testing results which can be used in communication efforts. Once your product is tested with our qPCR Smart Probes™ and has shown favourable results in supporting the microbiome, we can certify your product with our Maintains the Microbiome certification seal. We have ensured that our seal and certification are backed by quantifiable data and scientifically significant markers. The aim is to ensure our clients feel confident in making their claims and can communicate the true benefit of their microbiome formulations.

The Biome Mask has been created with the goal of maintaining the microbiome while targeting concerns such as skin dehydration and sensitivity. Introducing the Biome Mask™ Sequential Skin is proud to announce its biodegradable Biome Mask™ line designed to balance the skin microbiome and make your skin healthier. The Biome Mask™ line has five formulas to target five skin traits. Each is infused with serums that target specific concerns and uses prebiotics and postbiotics to balance the skin's microbiome. All masks have been tested in vivo. Made for all skin types Our formula, your benefits Semi-gel Tencel™ Versatile, soft & pleasant to the skin Eco-friendly Material Better for you and the planet 100% Cellulose Cellulose is the most abundant natural organic compound on Earth Remarkable Moisture Retention For long lasting benefits Click Here to see a Full List of our Ingredients How They Work Prebiotics Prebiotics beneficially affect the host by selectively stimulating the growth and/or activity of one or a limited number of bacteria on the skin, and thus improves host health. Feed your skin microbiome! Prebiotics act as good for the microbes on the skin, and allow good bacteria to grow and thrive. Probiotics Probiotics are living microorganisms that confer benefit to the host when applied to the body. Because probiotics are live bacteria, it is incredibly difficult to preserve and put into skincare products (especially when skincare contains preservatives). In fact, some products on the market that say they use probiotics, are actually using prebiotics or postbiotics. Postbiotics Postbiotics are the byproduct of fermented live bacteria (probiotics) or inactivated microorganisms. The process of fermentation releases nutrients inside of the bacteria and holds concentrated and nourishing skin health benefits that help to balance the skin microbiome. INGREDIENTS FROM MICROBIAL ORIGIN No INTENDED TO BE UTILISED BY THE HUMAN MICROBIOTA? No Yes Yes ARE THEY VIABLE? No Yes ORIGIN VIABILITY FUNCTION ORDINARY INGREDIENT ORDINARY INGREDIENT WITH PREBIOTIC FUNCTION INTENDED TO BE UTILISED BY THE HUMAN MICROBIOTA? PROBIOTIC INGREDIENT No Yes POSTBIOTIC INGREDIENT POSTBIOTIC INGREDIENT WITH PREBIOTIC FUNCTION Also available in a box of 5 Biome Masks™ Interested? What is Sequential's testing platform? Sequential has developed the gold standard test for microbiome-friendly products, in vivo (in, or on, humans). Finally, we can give some certainty about if a product is truly affecting the microbiome. We offer a complete end-to-end solution to support microbiome-friendly claims. From consultancy and study design to our proprietary microbiome testing kits. We analyse, interpret and report our findings to meet your needs. Why is it necessary to test the microbiome in vivo? At present, there are no regulations for microbiome-related formulas that brands and formulators can follow, however, it has been universally acknowledged that the in vivo method of conducting clinical studies is becoming critical and paramount to getting marketing claims through. When regulations are introduced, which may be imminent, the in vitro system will find itself lacking, resulting in limited claims and certifications that do not hold their value. This is why, we at Sequential strive to offer an in vivo approach, knowing full well that we want our client's claims to be significantly backed by scientific and quantifiable data. What type of sequencing technology does Sequential use for analysis? We offer four types of sequencing techniques including qPCR with our Smart Probes™, 16S, ITS and Shotgun Metagenomics. Using next-generation sequencing of the collection of microorganisms found on the body, during product usage, Sequential investigates the microbial diversity, and particular microorganisms we know are important and play a role in a healthy microbiome. Does Sequential offer claims certification for tested products? We provide our clients with a certification to claim “Maintains the Microbiome” subject to in vivo testing results which can be used in communication efforts. Once your product is tested with our qPCR Smart Probes™ and has shown favourable results in supporting the microbiome, we can certify your product with our Maintains the Microbiome certification seal. We have ensured that our seal and certification are backed by quantifiable data and scientifically significant markers. The aim is to ensure our clients feel confident in making their claims and can communicate the true benefit of their microbiome formulations.

What is a Skin Microbiome? The first layer of defense for your skin against outside forces is made up of good bacteria. A diverse microbiome means healthy skin. The secrets behind your skin microbiome On the surface of your skin sits an organic ecosystem made up from billions of good bacteria called the microbiome. It forms the first layer of defense for your skin against outside forces. In order for your skin microbiome to be healthy, it must be balanced. A balanced microbiome means healthy skin What affects your skin microbiome? Your environment The country you live in can have adverse effects on your skin microbiome, from pollution levels to UV exposure. Your skin care routine When it comes to skin care, not knowing what products to use can negatively impact your skin microbiome. Your physical wellbeing What you put in your body is as important as what you put on it. A healthy diet can affect your skin health. Your mental health When you aren’t well internally, it can be a struggle to look after yourself. It is imperative to be aware of your mental health. What is Sequential's testing platform? Sequential has developed the gold standard test for microbiome-friendly products, in vivo (in, or on, humans). Finally, we can give some certainty about if a product is truly affecting the microbiome. We offer a complete end-to-end solution to support microbiome-friendly claims. From consultancy and study design to our proprietary microbiome testing kits. We analyse, interpret and report our findings to meet your needs. Why is it necessary to test the microbiome in vivo? At present, there are no regulations for microbiome-related formulas that brands and formulators can follow, however, it has been universally acknowledged that the in vivo method of conducting clinical studies is becoming critical and paramount to getting marketing claims through. When regulations are introduced, which may be imminent, the in vitro system will find itself lacking, resulting in limited claims and certifications that do not hold their value. This is why, we at Sequential strive to offer an in vivo approach, knowing full well that we want our client's claims to be significantly backed by scientific and quantifiable data. What type of sequencing technology does Sequential use for analysis? We offer four types of sequencing techniques including qPCR with our Smart Probes™, 16S, ITS and Shotgun Metagenomics. Using next-generation sequencing of the collection of microorganisms found on the body, during product usage, Sequential investigates the microbial diversity, and particular microorganisms we know are important and play a role in a healthy microbiome. Does Sequential offer claims certification for tested products? We provide our clients with a certification to claim “Maintains the Microbiome” subject to in vivo testing results which can be used in communication efforts. Once your product is tested with our qPCR Smart Probes™ and has shown favourable results in supporting the microbiome, we can certify your product with our Maintains the Microbiome certification seal. We have ensured that our seal and certification are backed by quantifiable data and scientifically significant markers. The aim is to ensure our clients feel confident in making their claims and can communicate the true benefit of their microbiome formulations. Unearth the secrets of your skin microbiome We are skin experts We are a team of award-winning scientists with a mission to empower individuals to make better-informed decisions about their skin health. Our Story

Atopic dermatitis (AD), a chronic inflammatory skin condition, affects around 31.6 million people in the U.S., with 15-20% of children globally and 7.3% of U.S. adults experiencing the condition. TARGETED SKIN PANEL Atopic Dermatitis Targeted Panel 23 Smart Probes ™ Atopic Dermatitis Panel Validated targets: Staphylococcus aureus, Malassezia spp, Staphylococcus epidermidis Absolute Quantification End-to-end Service Qet a Quote Atopic Dermatitis Study with Sequential's Cutting-Edge Microbiome Testing Why Microbiome Testing for Atopic Dermatitis? Atopic dermatitis (AD), a chronic inflammatory skin condition, affects around 31.6 million people in the U.S., with 15-20% of children globally and 7.3% of U.S. adults experiencing the condition (National Eczema Association). AD often results from a combination of genetic predisposition and environmental triggers, such as irritants in cosmetic products. Disruptions to the skin’s microbiome play a significant role in the disease's development and severity. Imbalances in beneficial and harmful microbes can exacerbate AD, highlighting the importance of targeted treatments. In vivo testing for products aimed at managing AD is essential because it allows researchers to understand how formulations impact both the skin barrier and microbiome in real-world conditions. Properly tested products can help reduce irritation and avoid worsening symptoms, offering a science-backed approach to skincare for those with AD. Sequential is leading the way in microbiome testing for the industry. With a specialized focus on skin conditions, we have developed an innovative solution tailored to help you understand the root causes of AD at the microbial level. Our proprietary sequencing panel is designed specifically to offer in-depth analysis of AD-associated microbiota, giving you the tools to transform skincare solutions. Customized Analysis Tailored insights specific to AD-prone skin, allowing your R&D team to develop more personalized and effective products. Actionable Data Leverage detailed reports to guide formulation decisions and validate product efficacy with precision. Product Innovation New AD treatments or enhancing existing lines, our data helps you create targeted solutions with proven results. Precision Sequencing Target specific microbial communities associated with AD development and severity. Smart Probe s ™ Our dedicated team of scientists has developed a revolutionary method for evaluating microbes through our Smart Probes™. This proprietary panel includes 23 key microbes that we have specifically identified as having the most significant impact on skin health. To further understand the intricate relationship between atopic dermatitis (AD) and the skin microbiome, we use qPCR technology. This advanced technique allows us to precisely quantify microbial populations on the skin, providing deeper insights into how AD affects the microbiome. By closely monitoring how products interact with both beneficial and harmful microbes, companies can create solutions for AD but also restore a healthy balance to the skin’s ecosystem. Atopic Dermatitis Panel Targets Staphylococcus aureus This bacterium is frequently found in higher numbers on the skin of individuals with atopic dermatitis. It can exacerbate inflammation and contribute to skin barrier disruption. Malassezia spp This genus of yeast, which is part of the normal skin flora, can become overgrown in individuals with atopic dermatitis, potentially aggravating symptoms. Staphylococcus epidermidis While generally considered a commensal organism, imbalances in its population, along with other microbes, can influence the skin's health and contribute to atopic dermatitis symptoms. Industry Leader in Absolute Quantification Sequential’s Smart Probe ™ qPCR approach to microbiome analysis can resolve species and subspecies, giving statistically significant findings between skin conditions on in vivo human samples, compared to conventional sequencing approaches. Relevant Research Revolutionising Eczema Care: The Promise of Live Biotherapeutic Products AOB for AD: Ammonium-oxidising Bacteria as an Innovative Approach to Managing Atopic Dermatitis Answers for Atopic Dermatitis and Allergies: What is the Role of the Skin Bacteriome? Read More Articles

Find answers to common questions about Sequential's microbiome testing services for personal care products. Learn more about our process, benefits, and certifications. What is Sequential's testing platform? Sequential has developed the gold standard test for microbiome-friendly products, in vivo (in, or on, humans). Finally, we can give some certainty about if a product is truly affecting the microbiome. We offer a complete end-to-end solution to support microbiome-friendly claims. From consultancy and study design to our proprietary microbiome testing kits. We analyse, interpret and report our findings to meet your needs. Why is it necessary to test the microbiome in vivo? At present, there are no regulations for microbiome-related formulas that brands and formulators can follow, however, it has been universally acknowledged that the in vivo method of conducting clinical studies is becoming critical and paramount to getting marketing claims through. When regulations are introduced, which may be imminent, the in vitro system will find itself lacking, resulting in limited claims and certifications that do not hold their value. This is why, we at Sequential strive to offer an in vivo approach, knowing full well that we want our client's claims to be significantly backed by scientific and quantifiable data. What type of sequencing technology does Sequential use for analysis? We offer four types of sequencing techniques including qPCR with our Smart Probes™, 16S, ITS and Shotgun Metagenomics. Using next-generation sequencing of the collection of microorganisms found on the body, during product usage, Sequential investigates the microbial diversity, and particular microorganisms we know are important and play a role in a healthy microbiome. Does Sequential offer claims certification for tested products? We provide our clients with a certification to claim “Maintains the Microbiome” subject to in vivo testing results which can be used in communication efforts. Once your product is tested with our qPCR Smart Probes™ and has shown favourable results in supporting the microbiome, we can certify your product with our Maintains the Microbiome certification seal. We have ensured that our seal and certification are backed by quantifiable data and scientifically significant markers. The aim is to ensure our clients feel confident in making their claims and can communicate the true benefit of their microbiome formulations.

Video on how to take a microbiome sample with Sequential's microbiome testing kit.

Acne affects nearly 85% of people between the ages of 12 and 24, with millions worldwide continuing to struggle with breakouts into adulthood (American Academy of Dermatology). TARGETED SKIN PANEL Acne Targeted Panel 23 Smart Probes ™ Acne Panel Validated targets: Cutibacterium acnes acnes (subtype I) Cutibacterium acnes defendens (subtype II), Cutibacterium acnes elongatum (subtype III) Absolute Quantification End-to-end Service Qet a Quote Unlock the Secrets of Acne with Sequential's Cutting-Edge Microbiome Testing Why Microbiome Testing for Acne? Acne affects nearly 85% of people between the ages of 12 and 24, with millions worldwide continuing to struggle with breakouts into adulthood (American Academy of Dermatology). While many individuals turn to over-the-counter products in hopes of finding relief, the reality is that many of these formulations can exacerbate the problem. Without proper testing, products may contain ingredients that disrupt the delicate balance of the skin, leading to irritation, clogged pores, and even more severe acne. Acne isn’t just skin-deep — it’s driven by a delicate balance of microorganisms on the skin. As skincare companies race to meet demand, a lack of microbiome-focused testing means many products fail to address the root microbial causes of acne. Sequential is leading the way in microbiome testing for the industry. With a specialized focus on skin conditions, we have developed an innovative solution tailored to help you understand the root causes of acne at the microbial level. Our proprietary sequencing panel is designed specifically to offer in-depth analysis of acne-associated microbiota, giving you the tools to transform skincare solutions. Customized Analysis Tailored insights specific to acne-prone skin, allowing your R&D team to develop more personalized and effective products. Actionable Data Leverage detailed reports to guide formulation decisions and validate product efficacy with precision. Product Innovation New acne treatments or enhancing existing lines, our data helps you create targeted solutions with proven results. Precision Sequencing Target specific microbial communities associated with acne development and severity. Smart Probe s ™ Our dedicated team of scientists has developed a groundbreaking method for evaluating microbes through our Smart Probes™, a proprietary panel featuring 23 key microbes specifically identified as having the greatest impact on skin health. To further explore the intricate connection between acne and the skin microbiome, we employ qPCR technology. This cutting-edge technique allows us to precisely measure microbial populations on the skin, providing a clearer understanding of how imbalances in the microbiome can contribute to acne breakouts. By carefully analyzing how skincare products interact with both beneficial and acne-causing microbes, companies can create solutions that not only target acne symptoms but also support a healthier, balanced skin microbiome, promoting clearer and more resilient skin. Acne Panel Targets Cutibacterium acnes acnes (Subtype I) Cutibacterium acnes subtype I is a specific strain of the C. acnes bacterium commonly found within sebaceous (oil) glands. This bacterium plays a key role in maintaining skin health by breaking down sebum, and producing fatty acids that prevent harmful pathogens from colonizing. However, certain strains of subtype I are associated with inflammatory conditions like acne vulgaris. While typically harmless, its overgrowth or imbalance in the skin microbiome can trigger immune responses that lead to acne and other skin issues. Spittaels KJ, Ongena R, Zouboulis CC, Crabbé A, Coenye T. Cutibacterium acnes Phylotype I and II Strains Interact Differently With Human Skin Cells. Front Cell Infect Microbiol. 2020 Nov 16;10:575164. doi: 10.3389/fcimb.2020.575164. PMID: 33330124; PMCID: PMC7717938. Cutibacterium acnes defendens (Subtype II) Cutibacterium acnes subtype II is a strain of the C. acnes bacterium, distinct from subtype I, and is found on human skin, primarily in sebaceous areas. While it shares similarities with other subtypes in maintaining skin health by metabolizing sebum, subtype II is less commonly associated with acne development. It has been linked more to opportunistic infections and medical device-related infections rather than inflammatory acne. Subtype II is typically less inflammatory than subtype I and plays a different role in the skin's microbial balance. Cobian N, Garlet A, Hidalgo-Cantabrana C, Barrangou R. Comparative Genomic Analyses and CRISPR-Cas Characterization of Cutibacterium acnes Provide Insights Into Genetic Diversity and Typing Applications. Front Microbiol. 2021 Nov 3;12:758749. doi: 10.3389/fmicb.2021.758749. PMID: 34803983; PMCID: PMC8595920. Cutibacterium acnes elongatum (Subtype III) Cutibacterium acnes subtype III is a less common strain of the C. acnes bacterium, primarily found in deeper skin layers and associated more with opportunistic infections rather than acne. Unlike subtypes I and II, which are typically found on the skin's surface and sebaceous areas, subtype III is linked to post-surgical infections, particularly around medical implants, and is less involved in acne formation. It tends to have lower inflammatory potential, distinguishing it from other C. acnes subtypes in terms of both location and clinical relevance. Dagnelie MA, Khammari A, Dréno B, Corvec S. Cutibacterium acnes molecular typing: time to standardize the method. Clin Microbiol Infect. 2018 Nov;24(11):1149-1155. doi: 10.1016/j.cmi.2018.03.010. Epub 2018 Mar 12. PMID: 29544912. Industry Leader in Absolute Quantification Sequential’s Smart Probe ™ qPCR approach to microbiome analysis can resolve species and subspecies, giving statistically significant findings between skin conditions on in vivo human samples, compared to conventional sequencing approaches. Relevant Research Acne & The Skin Microbiome? Unveiling Nature's Acne Assassin: The Power of Essential Oils Benzoyl Peroxide & the Skin Microbiome Read More Articles

Taking a microbiome sample for the purpose of gene expression.