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Throughout the year, Sequential has the pleasure of going to various conferences and trade shows where we talk all things microbiome testing and clinical studies. Don't miss us when we're in your city next! Connect With Us At Conferences At Sequential, we believe in the power of personal connections and the exchange of ideas to foster innovation and growth. As we actively participate in various conferences and industry events, we invite you to be a part of these enriching experiences. Explore the opportunities to engage with our team, discover the latest industry trends, and strengthen the bonds that drive mutual success. Join us at conferences to connect, learn, and pave the way for future collaborations. Where we will be in 2025 22-23 January Pontoise, France 5-6 March Zhaoqing, China 18-19 March Brussels, Belgium 8-10 April Amsterdam, Netherlands 28-29 April The Hague, Netherlands 3-4 June New York City, USA 11-12 November Coventry, United Kingdom 5th Edition Comet Our Skincare Director, Petronille Houdart, will have the pleasure of attending this year's 5th Edition Comet Conference. 9th Microbiome R&D and Business Collaboration Congress: Asia We will be at this year's 9th Global Engage Conference in Asia, where our Co-Founder and CSO, Dr Albert Dashi, will also attend! Microbiome Times Partnering Forum We are incredibly excited to join the microbiome experts who will be speaking in Belgium this year! InCosmetics Global This spring, we have the pleasure of returning to In-Cosmetics Global Come speak with us at Booth 12C70! Skin Microbiome & Cosmeceuticals Congress: Europe An unforgettable must-attend congress for us, we look forward to seeing you at this year's Global Engage Congress! NYSCC Supplier's Day We are thrilled to add the New York Supplier's Day back into our rotation of conferences this year, we look forward to seeing you there! (Booth information TBA) SCS FORMULATE Come check out our booth (600) at this year's SCS Formulate and speak to us about all things microbiome! Connect with us on Socials!

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Sequential Skin has developed the Skin Microbiome Test Kit to discover the state of skin microbiomes. You can do Skin Health Check to find what your skin needs. Sequential Skin has developed the first of its kind skin health test to discover the state of individuals' skin microbiome. People can now deep dive into their Skin Profile to figure out what their skin needs. Test your skin microbiome Benefits of your Skin Profile Skin Health Understand the state of your skin through your Skin Microbiome Balance Index score Skin Traits Learn about your skin traits through our in-app quiz and solutions for healthier skin Skin Age Discover your skin age and whether or not your skin is older/younger than you are Environment Unearth how your physical environment is impacting your skin microbiome Sample Register your kit: Register your kit on our Skin Health Tracker mobile app and answer the in-app quiz. Our Testing Technology Our microbiome test is the most comprehensive test on the market. We use a method known as quantitative real-time PCR (qPCR) that looks at specific markers in your skin microbiome and how they impact your skin. Our microbiome markers were generated in an ISO 13485 (medical devices) and CAP-accredited lab, ensuring the quality, accuracy and reliability of our results. Skin Health Testing Kit Partner with Sequential Skin to bring our innovative skin health test to your customers. Give them an in-depth analysis of what lies on the surface of their skin and how best to increase its overall wellbeing. Register your Interest What is Sequential's testing platform? Sequential has developed the gold standard test for microbiome-friendly products, in vivo (in, or on, humans). Finally, we can give some certainty about if a product is truly affecting the microbiome. We offer a complete end-to-end solution to support microbiome-friendly claims. From consultancy and study design to our proprietary microbiome testing kits. We analyse, interpret and report our findings to meet your needs. Why is it necessary to test the microbiome in vivo? At present, there are no regulations for microbiome-related formulas that brands and formulators can follow, however, it has been universally acknowledged that the in vivo method of conducting clinical studies is becoming critical and paramount to getting marketing claims through. When regulations are introduced, which may be imminent, the in vitro system will find itself lacking, resulting in limited claims and certifications that do not hold their value. This is why, we at Sequential strive to offer an in vivo approach, knowing full well that we want our client's claims to be significantly backed by scientific and quantifiable data. What type of sequencing technology does Sequential use for analysis? We offer four types of sequencing techniques including qPCR with our Smart Probes™, 16S, ITS and Shotgun Metagenomics. Using next-generation sequencing of the collection of microorganisms found on the body, during product usage, Sequential investigates the microbial diversity, and particular microorganisms we know are important and play a role in a healthy microbiome. Does Sequential offer claims certification for tested products? We provide our clients with a certification to claim “Maintains the Microbiome” subject to in vivo testing results which can be used in communication efforts. Once your product is tested with our qPCR Smart Probes™ and has shown favourable results in supporting the microbiome, we can certify your product with our Maintains the Microbiome certification seal. We have ensured that our seal and certification are backed by quantifiable data and scientifically significant markers. The aim is to ensure our clients feel confident in making their claims and can communicate the true benefit of their microbiome formulations.

Rosacea is a chronic skin condition affecting an estimated 16 million Americans and millions more worldwide, primarily adults over 30 (National Rosacea Society). TARGETED SKIN PANEL Rosacea Targeted Panel 23 Smart Probes ™ Rosacea Panel Validated targets: Demodex mites, Bacillus oleronius, Helicobacter pylori Absolute Quantification End-to-end Service Qet a Quote Unlock the Secrets of Rosacea with Sequential's Cutting-Edge Microbiome Testing Why Microbiome Testing for Rosacea? Rosacea is a chronic skin condition affecting an estimated 16 million Americans and millions more worldwide, primarily adults over 30 (National Rosacea Society). Characterized by redness, visible blood vessels, and inflammation, rosacea can significantly impact an individual’s quality of life. Research suggests that the skin microbiome plays a crucial role in the development and severity of rosacea. Specifically, imbalances in microbial populations, such as an overgrowth of Demodex mites or harmful bacteria, can exacerbate symptoms. In vivo testing of skincare products for rosacea is essential to ensure that formulations are compatible with the unique microbiome of rosacea-prone skin. Such testing allows researchers to observe real-world effects on microbial balance and inflammation, ensuring that products do not unintentionally worsen symptoms. Without this careful testing, products may disrupt the microbiome further, leading to increased redness, irritation, or flare-ups. Sequential is leading the way in microbiome testing for the industry. With a specialized focus on skin conditions, we have developed an innovative solution tailored to help you understand the root causes of rosacea at the microbial level. Our proprietary sequencing panel is designed specifically to offer in-depth analysis of rosacea-associated microbiota, giving you the tools to transform skincare solutions. Customized Analysis Tailored insights specific to rosacea-prone skin, allowing your R&D team to develop more personalized and effective products. Actionable Data Leverage detailed reports to guide formulation decisions and validate product efficacy with precision. Product Innovation New rosacea treatments or enhancing existing lines, our data helps you create targeted solutions with proven results. Precision Sequencing Target specific microbial communities associated with rosacea development and severity. Smart Probe s ™ Our dedicated team of scientists has developed a revolutionary method for evaluating microbes through our Smart Probes™, a proprietary panel that includes 23 key microbes specifically identified as having the most significant impact on skin health. To further understand the complex relationship between rosacea and the skin microbiome, we utilize qPCR technology. This advanced method allows us to accurately quantify microbial populations on the skin, offering deeper insights into how rosacea affects the balance of beneficial and harmful microbes. By closely monitoring how skincare products interact with these microbes, companies can develop solutions that not only target rosacea symptoms but also help restore a healthier, balanced skin microbiome, providing long-term relief for sufferers. Rosacea Panel Targets Demodex mites (Demodex folliculorum and Demodex brevis) These tiny mites are normally present on the skin but are often found in higher numbers on the skin of individuals with rosacea. Their presence is thought to trigger inflammatory responses. Forton FMN. The Pathogenic Role of Demodex Mites in Rosacea: A Potential Therapeutic Target Already in Erythematotelangiectatic Rosacea? Dermatol Ther (Heidelb). 2020 Dec;10(6):1229-1253. doi: 10.1007/s13555-020-00458-9. Epub 2020 Oct 23. PMID: 33095403; PMCID: PMC7649190. Kim HS. Microbiota in Rosacea. Am J Clin Dermatol. 2020 Sep;21(Suppl 1):25-35. doi: 10.1007/s40257-020-00546-8. PMID: 32914214; PMCID: PMC7584533. Bacillus oleronius This bacterium has been linked to rosacea, particularly in relation to the inflammatory response associated with the condition. It is often found in higher quantities on the skin of rosacea patients. Helicobacter pylori Although not a skin microbe per se, this bacterium, commonly associated with gastrointestinal issues, has been implicated in some studies as a potential contributor to rosacea flare-ups or exacerbations. Yang X. Relationship between Helicobacter pylori and Rosacea: review and discussion. BMC Infect Dis. 2018 Jul 11;18(1):318. doi: 10.1186/s12879-018-3232-4. PMID: 29996790; PMCID: PMC6042414. Industry Leader in Absolute Quantification Sequential’s Smart Probe ™ qPCR approach to microbiome analysis can resolve species and subspecies, giving statistically significant findings between skin conditions on in vivo human samples, compared to conventional sequencing approaches. Relevant Research How Does the Skin Microbiome Influence Rosacea? Unveiling the Microbial Puzzle Read More Articles

Discover the power of combining microbiome testing and clinical data. Elevate your understanding with our comprehensive Clinical Assessments. Clinical Assessments for More In-Depth Analysis When it comes to testing your personal care products, we offer add-ons to supplement your microbiome research efforts and facilitate your testing experience. It has become clear in recent years that differentiation is key when creating a formulation for the competitive market, however, differentiation can be incredibly straightforward when backed with the data and scientific evidence to support it. Not only will you differentiate your brand, you will also demonstrate your commitment to providing leading-edge and effective solutions for your customers’ personal care and skincare needs. Start Your Biophysical Study Today! The only way to understand how a product performs is by putting it to the test Hydration Measurement (Corneometry and TEWL) Gain a comprehensive understanding of your product's impact on moisture retention, skin hydration and overall skin barrier function. This data can guide the development of effective moisturizing formulations. Perception Questionnaire Uncovering how users experience and interact with products. These surveys offer valuable insights into subjective factors like texture, scent, and overall satisfaction ensuring that your scientific data is enriched. Elasticity Measurement (Elastometer) Unravel the effect your product has on the skin’s elasticity through the use of elastometers, allowing you to position your product within a firming category with demonstrated benefits for graceful aging. High-Resolution Imaging Use before and after images to provide visual evidence of a product’s impact on the skin’s appearance. These tangible comparisons offer brands the ability to objectively assess changes in the skin. Dermatologist Assessment Measure and quantify inflammatory disease, atopic dermatitis (through SCORAD), psoriasis (through PASI), comedogenicity, and other hallmarks of skin conditions, by our board-certified and in-house dermatologists. Skin pH Measurement Maintain the delicate balance of the skin's acid mantle with your product by measuring pH to ensure compatibility and minimise the risk of irritation for a gentle, biome supporting formulation. FAQ What is Sequential's testing platform? Sequential has developed the gold standard test for microbiome-friendly products, in vivo (in, or on, humans). Finally, we can give some certainty about if a product is truly affecting the microbiome. We offer a complete end-to-end solution to support microbiome-friendly claims. From consultancy and study design to our proprietary microbiome testing kits. We analyse, interpret and report our findings to meet your needs. Why is it necessary to test the microbiome in vivo? At present, there are no regulations for microbiome-related formulas that brands and formulators can follow, however, it has been universally acknowledged that the in vivo method of conducting clinical studies is becoming critical and paramount to getting marketing claims through. When regulations are introduced, which may be imminent, the in vitro system will find itself lacking, resulting in limited claims and certifications that do not hold their value. This is why, we at Sequential strive to offer an in vivo approach, knowing full well that we want our client's claims to be significantly backed by scientific and quantifiable data. What type of sequencing technology does Sequential use for analysis? We offer four types of sequencing techniques including qPCR with our Smart Probes™, 16S, ITS and Shotgun Metagenomics. Using next-generation sequencing of the collection of microorganisms found on the body, during product usage, Sequential investigates the microbial diversity, and particular microorganisms we know are important and play a role in a healthy microbiome. Does Sequential offer claims certification for tested products? We provide our clients with a certification to claim “Maintains the Microbiome” subject to in vivo testing results which can be used in communication efforts. Once your product is tested with our qPCR Smart Probes™ and has shown favourable results in supporting the microbiome, we can certify your product with our Maintains the Microbiome certification seal. We have ensured that our seal and certification are backed by quantifiable data and scientifically significant markers. The aim is to ensure our clients feel confident in making their claims and can communicate the true benefit of their microbiome formulations.

Sequential is honoured to be spoken and written about in numerous publications. We are always keen to speak about the great milestones we are hitting with our personal care microbiome testing platform. Sequential in the Press Sequential and AMILI secure $1.8M UK-Singapore R&D project to advance gut-skin microbiome science 13-Feb-2025 Read Now New clinical testing to “investigate microbial roots of various skin conditions” 17-Oct-2024 Read Now Losing its meaning? ‘Microbiome-friendly’ terms at risk of misleading consumers – Sequential CEO 29-Jul-2024 Read Now Navigating the realm of intimate care products 17-Jan-2024 Read Now Avec ce test microbiome maison, 10 secondes m'ont suffit pour découvrir les besoins de ma peau 04-Oct-2023 Read Now Lysine-Dendrimer, a New Non-Aggressive Solution to Rebalance the Microbiota of Acne-Prone Skin 3-Aug-2023 Read Now Sequential Announces New Collaboration Agreement to Measure Skin Health for Leading Developer of Personal Care Products 15-Mar-2023 Read Now Sequential Skin partners with Johnson & Johnson on microbiome tests 10-Mar-2023 Read Now Symbiome Partners with Skin Microbiome Testing experts at Sequential to Revolutionize Beauty Industry 26-Jan-2023 Read Now Sequential Skin Nabs $500K UK Grant to Study Skin Microbiome in Atopic Dermatitis 29-Jun-2022 Read Now Demands rising for clinical data on the skin microbiome: Experts 22-Mar-2022 Read Now ‘Maintaining the microbiome’: Biotech startup Sequential Skin debuts in vivo B2B testing service 03-Mar-2022 Read Now Start-up Sequential Skin bags $1.65m of fresh funding 12-Oct-2021 Read Now Singapore Beauty Startup Sequential Skin Uses Science to Help Identify the Best Skincare For You 07-Jan-2021 Read Now Overlooked opportunities? Skin microbiome may play a more important role in personalisation than DNA 13-Aug-2020 Read Now Sequential tool kit prioritises personalisation 15-Jan-2020 Read Now Sequential and AMILI Secure Prestigious $1.8M UK-Singapore Collaborative R&D Project 13-Feb-2025 Read Now Sequential Launches Industry-First Microbiome Target Panels for Skin Conditions 16-Oct-2024 Read Now From the experts: Top 3 trends in skin microbiome research 20-Jun-2024 Read Now Is Home-Based Skin Microbiome Testing Transforming Beauty and Health Care Industry? 7-Dec-2023 Read Now “Hormone-led changes cause differences to the vaginal ecology that can have an effect on women’s health later in life.” CEO of Sequential 13-Sep-2023 Read Now Sequential and Johnson & Johnson enter partnership to develop new genomic-based skin test 16-Mar-2023 Read Now Sequential Announces New Collaboration Agreement to Quantify Skin Health 15-Mar-2023 Read Now ‘Natural evolution’: Gallinée unveils at-home skin microbiome test kits with Sequential Skin 09-Mar-2023 Read Now Here They Are: Congratulations to the Winners of the 2022 C&T Allēs 29-Sep-2022 Read Now Sequential Skin: Why We Invested 11-Apr-2022 Read Now Sequential Skin Debuts Sequential Bio Testing Solution 09-Mar-2022 Read Now Biotech Start-Up Sequential Skin Raises Funds 10-Dec-2021 Read Now Biotech startup Sequential Skin raises €1.4m – ‘We want to accelerate the future of skin health,’ says CEO 11-Oct-2021 Read Now PODCAST – Holistic view on skin: Sequential Skin on why microbiome testing is crucial to personalised beauty 21-Aug-2020 Read Now Beauté personnalisée: Sequential Skin la Beauty Tech made in Singapore 07-Jul-2020 Read Now GWS 2019 DAY 2 Beauty, Science & Technology: Skin in the Game 25-Nov-2019 Read Now 50694 Gut microbiome differences between patients with moderate to severe Chronic Hand Eczema and healthy subjects Sep-2024 Read Now Is Microbiome Certification even Necessary? 29-Jul-2024 Read Now ‘It’s a really delicate system’: Research on vaginal microbiome shows how little we know about femcare 22-Jan-2024 Read Now Gallinée lance le premier test de santé de la peau et du microbiome à réaliser chez soi 10-Oct-2023 Read Now Lysine-dendrimer: a gentle yet effective acne treatment that maintains skin microbiome, says study 18-Aug-2023 Read Now Acne and ageing: J&J exploring skin health markers via Sequential Skin research tie-up 15-Mar-2023 Read Now Sequential Skin x Johnson & Johnson Collab to Quantify Skin Health 14-Mar-2023 Read Now Understand before purchase: Microbiome testing can help consumers find the right products and eliminate waste 30-Jan-2023 Read Now Sequential Wins New Grant To Propel Skin Microbiome Research 30-Jun-2022 Read Now Sequential Skin launches test to assess ingredient effect on microbiome 22-Mar-2022 Read Now Microbiome start-up Sequential Bio debuts first in-vivo testing for personal care industry 04-Mar-2022 Read Now Sequential Skin Raises $1.6M in Oversubscribed Seed Round 12-Oct-2021 Read Now The skin microbiome brainstorm: Tech, claims and trend-driven NPD 16-Sep-2021 Read Now Skin Sensors Enable Personalized Skin Care 20-Aug-2020 Read Now Sequential Skin: beauty tech’s new approach to genetic analysis and microbiome skincare 09-Mar-2020 Read Now How genetic insights can personalise skin microbiome launches 29-Jul-2019 Read Now

Sequential is pleased to announce the opening of it's first European lab based in London. We look forward to bringing our clinical microbiome testing service for personal care products to the UK and greater European market. Unveiling Sequential Bio’s New London Lab testing HQ 18th October 2022 - In an effort to keeping the entire testing process in-house, allowing for rigorous quality control, and meeting the demand of our European clients - today we announce the opening of our testing lab in London. Sequential is proud to announce that it will open a European HQ, and testing lab site in White City, London. One key quality that makes Sequential stand out from its competitors is keeping the testing protocols and quantitative analysis in-house. This gives them the ability to ensure the processing, and targeting of microbiome markers for their clients is extremely high quality. “Innovative R&D, and rigorous quality control on our lab testing and processes have always been front of mind at Sequential. By opening a lab in London, we’re responding to the demand from customers - whilst retaining control over our quality in testing.” - Dr Oliver Worsley, CEO/Co-Founder. Adding to their testing labs in Singapore, and New York, in which they serve Singapore, Thailand, Australia, and the US markets. This enables faster turnaround time for European clients, as the demand for non-invasive molecular analysis of the skin reaches new heights in Europe. Between 2018 - 2022, there have been over 34 microbiome deals (licensing and investments) made. With an average contract value of $257M, this has created over $2.3B of value in the gut and skin microbiome space. Europe is a significant player in the global microbiome field, expected to grow at a CAGR of 24.25% (between 2022-2028). About Sequential Sequential is part of the Sequential Skin group – with a US lab in New York City, alongside a lab in London and a lab in Singapore. Sequential Bio team has over 20 years of combined expertise in genetics, epigenetics, and microbiome research. It was recognised recently as the “Most Significant ” Testing Method in the Industry. Sequential has validated its AI-driven testing platform with over 30 companies, analyzing over 12,000 skin microbiome samples. They specialise in skin, scalp and vulva/vaginal microbiome samples. They are supported by Enterprise SG, A*STAR, Genome Institute of Singapore, IndieBio New York, SOSV, Metaplanet Holdings, Scrum Ventures, Genedant VC, Ben Holmes (ex. General Partner at Index Ventures), Innovate UK, and are a resident company of Johnson & Johnson Innovation – JLABS.

Certify your intimate care product with us! Maximize microbiome benefits in your strategy. Let's get started today for success! Vaginal & Vulvar Microbiome Testing As the intimate care industry grows, consumers demand transparency from the brands formulating their products. It has become essential for formulators to create products they are willing to test to present scientifically backed data-driven evidence of their products' true effects. Sequential offers microbiome testing for your intimate care formulations, hygiene washes and other products geared towards that delicate area. We are dedicated to understanding how your product interacts with the vaginal region and its microbes. Depending on how in-depth you want to go, we offer qPCR, 16S, ITS, and Shotgun Metagenomics. Download Case Study! Personalized Approach to Testing Unlike other methodologies present within the industry, Sequential's approach ensures that your product's data and analysis will stand the test of industry regulations when they are introduced. You can tailor your study entirely to your unique requirements. Test Products in a Real-Life Context The microbiome comprises a complex ecosystem of microorganisms that live together in a delicate balance, which is why it's best to test directly upon it directly. To fully understand the impact a product is having on the microbiome, in vivo is the only way. Collect Longitudinal Data With in vivo testing we can design your study around the extended use of a product over multiple time points. This allows us to review how a product is performing before and after usage, but also take into account its gradual impact on the microbiome. Measure Against a Control Group Measure a product against a control group that might have a different percentage of your active ingredient within its formulation or no active at all. This will allow for deeper insights into the impact of a formulation on microbial balance and diversity. 4 Sequencing Reports To Pick From Depending on your development stage and what you are interested in studying we offer qPCR, 16S, ITS, and Shotgun Metagenomics. With our qPCR Smart Probes™ we can go down to the strain level in our analysis. Personalize Your Microbiome Study! The vaginal microbiome is a dynamic ecosystem primarily dominated by Lactobacillus bacteria, which produce lactic acid and help maintain an acidic pH, creating a hostile environment for potential pathogens. This acidic environment is crucial for preventing infections and promoting overall vaginal health. Smart Probe s ™ Our dedicated team of scientists have developed a method of evaluating microbes through our Smart Probes ™ . These refer to a panel of 20 key microbes we have specifically identified as having the most impact on vaginal health. Over and above the taxonomic characterisation that 16S offers, which gives us a snapshot of all the genus present within a collected sample (Lactobacillus , Streptococcus , etc.) our targeted approach takes it a step further, opening the lens to the species (L.crispatus ), sub-species and even strains within them. This is a crucial distinction as not all strains of a species behave similarly. In fact, we find that within these species there are strains associated with inflammation and strains that are commensal, and beneficial. Gold Standard Certification Sequential has developed the gold standard test for products designed to target the microbiome, in vivo (in, or on, humans). Finally, we can give some certainty about if a product is truly affecting the microbiome. Using next-generation sequencing of the collection of micro-organisms found on the body, before and after product usage, Sequential investigates the microbial balance and diversity, and particular micro-organisms we know are important and play a role in a healthy microbiome. We give you an in vivo certification that your product maintains the microbiome. And it’s not exclusive to skincare! We do this for haircare products, oral products, and vulva/vaginal microbiomes. Personalize Your Microbiome Study! Supplement Your Microbiome Study Recruitment Services Let us take care of the entire candidate recruitment process for you! View More Biophysical Assessments Increase your data on the use of your product by evaluating additional biophysical factors. View More Formulation Support Seek consultation advice for your formulation if you are re-formulating or developing a new product. View More FAQ What is Sequential's testing platform? Sequential has developed the gold standard test for microbiome-friendly products, in vivo (in, or on, humans). Finally, we can give some certainty about if a product is truly affecting the microbiome. We offer a complete end-to-end solution to support microbiome-friendly claims. From consultancy and study design to our proprietary microbiome testing kits. We analyse, interpret and report our findings to meet your needs. Why is it necessary to test the microbiome in vivo? At present, there are no regulations for microbiome-related formulas that brands and formulators can follow, however, it has been universally acknowledged that the in vivo method of conducting clinical studies is becoming critical and paramount to getting marketing claims through. When regulations are introduced, which may be imminent, the in vitro system will find itself lacking, resulting in limited claims and certifications that do not hold their value. This is why, we at Sequential strive to offer an in vivo approach, knowing full well that we want our client's claims to be significantly backed by scientific and quantifiable data. What type of sequencing technology does Sequential use for analysis? We offer four types of sequencing techniques including qPCR with our Smart Probes™, 16S, ITS and Shotgun Metagenomics. Using next-generation sequencing of the collection of microorganisms found on the body, during product usage, Sequential investigates the microbial diversity, and particular microorganisms we know are important and play a role in a healthy microbiome. Does Sequential offer claims certification for tested products? We provide our clients with a certification to claim “Maintains the Microbiome” subject to in vivo testing results which can be used in communication efforts. Once your product is tested with our qPCR Smart Probes™ and has shown favourable results in supporting the microbiome, we can certify your product with our Maintains the Microbiome certification seal. We have ensured that our seal and certification are backed by quantifiable data and scientifically significant markers. The aim is to ensure our clients feel confident in making their claims and can communicate the true benefit of their microbiome formulations.

What is a Microbiome Formulation? What is a Microbiome Formulation? Less is More approach Statistically, Women typically apply around 16 beauty products each day, exposing their skin to about 515 synthetic chemicals daily. (“The Average Woman Uses 16 Beauty Products Every Day. Here Are the Ones Worth the Investment,” 2018) This extensive exposure has led to a rise in skin sensitivity, which now affects 60-70% of women and 50-60% of men worldwide (Sensitive skins wear their own skin microbiota, 2024) - a number that continues to climb. To mitigate these effects, experts advise using fewer, essential ingredients and prioritising simple, balanced formulations over emphasising single "hero" ingredients. This holistic approach fosters skin compatibility, supporting healthier skin with reduced risk of irritation. Traditional vs Microbiome approach The traditional skin care approach highlights a single, "hero" ingredient or a blend of active ingredients to deliver specific benefits, with the spotlight on these components as the main drivers of product efficacy. However, a microbiome-focused approach takes a broader, more holistic perspective. It considers how each ingredient, including non-active additives (functional ingredients), interacts with the skin and its microbiome. This method aims to create formulations that support the skin's natural microbial balance, ensuring that every component contributes positively to overall skin health. Anhydrous Products Anhydrous products, with no water, do not require preservatives since bacteria need moisture to grow. However, they face challenges: incorporating water-soluble actives is difficult, and they are more prone to oxidation and rancidity. Their thick, oil-based consistency may also not suit all skin types, especially oily or sensitive skin. Despite these drawbacks, anhydrous formulations appeal to consumers interested in preservative-free, minimal skincare. Water-based Products Water in skincare products often makes up 80%, requiring emulsifiers to mix with oils and preservatives to prevent bacterial and fungal growth. To avoid contamination, effective preservation is essential. Instead of skipping preservation, reducing water activity can enhance product stability by limiting moisture, which is crucial for safer, less chemically intensive formulations. pH Maintaining a skincare formulation with a slightly acidic pH, ideally below 5, supports the skin's microbiome and barrier function. Alkaline cleansers with pH levels above 7 can disrupt the acid mantle, weakening the barrier and encouraging harmful bacteria. Hülpüsch et al. (2022) found that a higher skin pH (5.7–6.2) in atopic dermatitis patients is linked to increased Staphylococcus aureus colonisation, worsening inflammation and barrier issues. Acidic formulations may help control bacterial overgrowth, reducing flare-ups and promoting skin health in at-risk groups like those with AD. Ingredients Formulations that are high in lipids can strengthen the skin barrier, enhancing hydration and resilience against irritants. Combining gentle surfactants with super fatty agents further reduces potential for irritation and maintains moisture, which is particularly beneficial for sensitive skin types. These principles help prevent disruption of the microbiome, ensuring the skin remains balanced and resilient over time (Van Belkum et al., 2023). The selection of mildly acidic, pH-balanced ingredients close to the skin’s natural acidity (4.7 < pH < 5.7) is crucial, as it helps preserve the skin’s barrier and microbiome. Preservatives To protect the skin microbiome and reduce bacterial growth, it’s essential to minimise preservatives, especially in oil-based products. Anhydrous formulations, which don’t require preservatives, are ideal when possible. For water-based products, alternatives such as antimicrobial peptides (AMPs), and natural humectants like glycerin, sodium lactate, and NMF components (ceramide, urea), can improve microbial stability and skin barrier function (Halla et al., 2018). Airless packaging and sterilisation methods like UHT technology can further enhance product safety by minimising contamination, reducing the need for traditional preservatives. Surfactants Water-in-oil System: Choose emulsifiers with a low hydrophilic-lipophilic balance ratio and natural ingredients like plant oils or sugars. Limit emulsifier concentration to maintain skin barrier integrity. Avoid Polysorbate 80, which can promote pathogen growth, and use alternatives like Polyglyceryl 4 Oleate, which self-emulsify when mixed with water. Oil-in-water System: Use microbiome-friendly emulsifiers derived from natural sources like plant oils and sugars. Examples include Olivem 1000, made from olive oil (Cetearyl Olivate and Sorbitan Olivate), and Emulium Mellifera MB, which combines beeswax and jojoba wax (Polyglyceryl-6 Distearate and Jojoba Esters). These emulsifiers help maintain skin health while supporting the microbiome. It's also important to limit the concentration of emulsifiers to avoid disrupting the skin barrier. Fragrances/Essential Oils Fragrances should generally be avoided in skincare formulations, especially for sensitive skin, as many fragrances can cause irritation. However, certain essential oils may serve as antimicrobial agents in products for non-sensitive skin, offering a natural alternative to synthetic preservatives. For instance, bergamot and lavender oils demonstrate antibacterial and antifungal effects, particularly against Staphylococcus aureus, though they do not affect S. epidermidis (Kim et al., 2022). Additionally, rosemary oil and phenylethyl alcohol show strong antifungal properties, adding preservative benefits without traditional preservatives. Reference Byrd, A. L., Belkaid, Y., & Segre, J. A. (2018). The human skin microbiome. Nature Reviews Microbiology, 16(3), 143-155. Halla, N., Fernandes, I. P., Heleno, S. A., Costa, P., Boucherit-Otmani, Z., Boucherit, K., Rodrigues, A. E., Ferreira, I. C. F. R., & Barreiro, M. F. (2018). Cosmetics Preservation: A Review on Present Strategies. Molecules, 23(7), 1571. https://doi.org/10.3390/molecules23071571 The average woman uses 16 beauty products every day. Here are the ones worth the investment. (2018, December 6). The Telegraph. https://www.telegraph.co.uk/beauty/face/essential-skincare-makeup-products-use-everyday/ Sensitive skins wear their own skin microbiota - BEAUTY HORIZONS 1 2021 WW. (2024, March 27). https://digital.teknoscienze.com/beauty_horizons_1_2021_ww/sensitive_skins_wear_their_own_skin_microbiota Hülpüsch, C., Tremmel, K., Hammel, G., Bhattacharyya, M., De Tomassi, A., Nussbaumer, T., Neumann, A. U., Reiger, M., & Traidl‐Hoffmann, C. (2020). Skin pH–dependent Staphylococcus aureus abundance as predictor for increasing atopic dermatitis severity. Allergy, 75(11), 2888–2898. https://doi.org/10.1111/all.14461 Van Belkum, A., Lisotto, P., Pirovano, W., Mongiat, S., Zorgani, A., Gempeler, M., Bongoni, R., & Klaassens, E. (2023). Being friendly to the skin microbiome: Experimental assessment. Frontiers in Microbiomes, 1. https://doi.org/10.3389/frmbi.2022.1077151 Nielsen, H. L., et al. (2016). Influence of emulsifiers on microbial stability in cosmetic formulations. International Journal of Cosmetic Science, 38(4), 357-366. https://doi.org/10.1111/ics.12301 Kim, J., et al. (2022). Antimicrobial effects of essential oils on skin microbiota. Journal of Cosmetic Science, 73(5), 452-460.

The ingredient list for our Biome Mask formulation with each of their applications from giving deep hydration to protecting from pollutants with antioxidant benefits. Category Ingredient What is it? Benefit Melanin Targeters Arbutin Arbutin is an antioxidant that is extracted from different berries. Arbutin helps in ensuring that skin is brightened and pigmentation is reduced in the skin. It can also fade scars. Hydrators Squalane Squalane is a hydrogenated version of squalene. It is an emollient and behaves similar to our own skin sebum. Prevents moisture loss, can neutralize UV damage . Postbiotics Lactobacillus ferment Lactobacillus ferment is a non-living probiotic ingredient made by fermentation of the microorganism Lactobacillus. Thanks to its soothing effect on the skin, it helps to strengthen the skin's defense and maintains the health of the skin microbiome. Allantoin Allantoin is an extract from comfrey plant. Allantoin protects and soothes the skin. Trehalose Trehalose is a disaccharide with hydrating properties for the skin. It’s a plant sugar. Trehalose is used as a prebiotic and is effective to help maintain healthy skin. Green Tea Extract Extract from the leaves of green tea. Tea Extracts is an antioxidant ingredient that aids soothing the skin and can also address skin discoloration and early signs of aging. Colloidal Oatmeal Colloidal Oatmeal is cocktail of oats that has been refined into powder and acts as a skin protectant. Colloidal Oatmeal aids in locking in moisture in the skin barrier and also has soothing properties. Skin Soothing Multi-Ceramide [Ceramide NP; Phytosphingosine] Ceramides are lipids or fats that exist in skin cells and make up to 40% of your outer skin layer. Ceramides are crucial in aiding your skin to retain moisture and prevent unwanted bacteria from entering your body. Skin Repairing Peptide Complex [copper tripeptide-1, Acetyl heaxapeptide-8, Palmitoyl Pentapepdide-4, Palmitoyl tripeptide-1, Palmitoyl tetrapepide-7, tripeptide-1, tripeptide-2, tripeptide-2, tripeptide-3, acetyl Octapeptide 3] Peptides are amino acid chains that aid in the forming of certain types of protein in the skin such as elastin and collagen. Peptides can help in increasing skin elasticity and reducing signs of aging, allowing skin to appear firmer. Skin Shields Vitamin E [Tocopherol] Tocopherol is an excellent example of a powerful antioxidant that is oil-soluble, meaning it can easily dissolve into the skin. Tocopherol helps in combating free-radical damage (such as pollution), and can also be an effective hydrator. Retinyl Palmitate Retinyl Palmitate is the most abundantly used form of retinol (Vitamin A). Retinyl Palmitate has anti-aging properties as well as anti-oxidant properties. Centella Asiatica Centella Asiatica is a medicinal herb that is known to treat inflammation and has antioxidant properties. Centella Asiatica is used as an active ingredient in skincare products for anti-inflammation, anti-oxidant and soothes compromised skin. Sorbitol Sorbitol is a sugar with hydrating properties that can nourish the probiotics that live on the skin allowing the skin microbiome to be balanced. Sorbitol breakdown by microorganisms on the skin creates beta-glucan, a skin calming antioxidant. Skin Barrier Protectors (Hydrators) Hyaluronic Acid Hyaluronic Acid is a humectant, meaning that it attracts water from the air or within the skin. Hyaluaronic Acid is one of the most common known ingredients for increasing overall moisture in skin. Vitamin C [Ascorbic Acid] Ascorbic Acid is an antioxidant and the most stable Vitamin C form. Ascorbic Acid aids in brightening the skin and aids in the production of collagen to prevent premature aging. Prebiotics Fructooligosaccharides Fructooligosaccharides is a prebiotic sugar used as food by the skin microbiota. It’s also a good source of antioxidants. It helps to keep the skin clean, hydrated, and well nutrified. It helps to maintain a healthy microbiome for your skin. Niacinamide Niacinamide is a vitamin that is water-soluble and penetrate into the skin with ease. Niacinamide aids in reducing the visibility of pores and removes dullness and restores the skin. Licorice Root Licorice Root is a plant based ingredients that can be cultivated in Asia, Europe, and in the Middle East. Licorice Root is a brightening agent in skincare products and can repair the skin. Lactic Acid Lactic Acid is a metabolic by-product released by the fermentation of microorganisms like lactobacillus. Lactic acid strengthens the skin barrier and has a positive impact on the skin microbiome balance. It also helps in reducing uneven tone and brightens skin tone.

What Is a Skin Microbiome Kit? What Is a Skin Microbiome Kit? A skin microbiome test kit is a diagnostic tool designed to analyse the microorganisms living on the surface of the skin. These kits assess the diversity and abundance of bacteria, fungi, and other microbes, providing insights into the skin’s microbial ecosystem. The information gathered can help individuals and healthcare providers understand microbial imbalances that might contribute to dermatological conditions or identify strategies to improve skin health. Components of a Skin Microbiome Kit Sampling Materials Swabs: Sterile swabs are used to collect skin samples from specific areas, such as the face, hands, or scalp. Adhesive Strips: In some kits, tape-stripping methods collect deeper microbial samples by gently removing skin cells along with surface microbes. Sample Preservation The collected samples are placed into a vial or test tube containing a stabilising agent to preserve the DNA of microbes until the sample reaches the laboratory. Instructions for Use Clear instructions are included to guide users on how to collect samples correctly to avoid contamination and ensure accurate results. Once the sample arrives at the lab, it undergoes next-generation sequencing (NGS) or polymerase chain reaction (PCR) analysis. These techniques identify microbial DNA and quantify the presence of various microorganisms. (Nafea et al., 2024) Results are typically shared in reports, offering detailed insights into the composition of the user’s skin microbiome and recommendations for skincare or lifestyle adjustments. Applications and Benefits -Personalised skincare: Tailored treatments based on an individual’s skin microbial profile. By identifying specific bacteria or fungi, users can specifically customise their skincare routines, using products designed to promote a healthy microbiome or address specific concerns such as acne or dryness. Companies may also use the microbiome data collected to formulate more effective skincare products in the future. -Identifying dysbiosis: Kits can detect microbial imbalances (dysbiosis), which are associated with conditions such as eczema, acne, and psoriasis. (Borrego-Ruiz & Borrego, 2024) This allows dermatologists to recommend targeted therapies, such as probiotics or prebiotics, to restore balance and improve skin health. (D’Auria et al., 2021) They are also helpful for tracking the effectiveness of treatments over time. -Monitoring the impact of lifestyle changes: The skin microbiome is influenced by environmental factors like humidity, pollution, and UV exposure, as well as lifestyle choices such as diet and stress management. Testing allows users to monitor how these factors affect their microbial health and make adjustments accordingly. (Byrd et al., 2018) -Research purposes: Helping dermatologists and researchers understand the dynamics of skin health better and test the effectiveness of new treatments. This data can drive the development of these new therapies, including microbiome-based interventions, and help identify potential biomarkers for skin disorders. (D’Auria et al., 2021) Scientific Significance The skin microbiome plays a crucial role in maintaining the skin's barrier function and immune responses. Imbalances in these microbial communities are often associated with dermatological conditions, and research continues to explore how restoring balance through topical or oral interventions could improve skin health. Accurate sampling and analysis, however, remain critical challenges for researchers, requiring reproducible methods for reliable results. (D’Auria et al., 2021) Conclusion A skin microbiome kit offers a gateway into understanding the complex ecosystem on our skin. While still emerging as a mainstream tool, these kits are becoming popular in both consumer skincare and clinical research. As more studies focus on the interplay between the skin microbiome and external factors such as diet and environmental exposures, personalised skincare routines may soon become the norm. References Byrd, A. L., Belkaid, Y., & Segre, J. A. (2018). The human skin microbiome. Nature Reviews Microbiology, 16(3), 143-155. https://doi.org/10.1038/nrmicro.2017.157 Borrego-Ruiz, A., & Borrego, J. J. (2024). Microbial Dysbiosis in the Skin Microbiome and Its Psychological Consequences. Microorganisms, 12(9), 1908. https://doi.org/10.3390/microorganisms12091908 D'Auria, E., Acunzo, M., et al. (2021). A journey on the skin microbiome: Pitfalls and opportunities. International Journal of Molecular Sciences, 22(18), 9846. https://doi.org/10.3390/ijms22189846 Nafea, A. M., Wang, Y., Wang, D., Salama, A. M., Aziz, M. A., Xu, S., & Tong, Y. (2024). Application of next-generation sequencing to identify different pathogens. Frontiers in Microbiology, 14. https://doi.org/10.3389/fmicb.2023.1329330

< Back Sales Associate East Coast of the United States, USA Job Type Full Time Workspace Hybrid About the Role As a Sales Associate at Sequential, you will be essential in building relationships and expanding our reach within the microbiome industry. Your role will involve supporting business development efforts, assisting in sales strategy execution, and helping grow market share for Sequential’s innovative products and services. You will work closely with the Sales Director and team to meet sales goals, develop client relationships, and contribute to Sequential’s mission of advancing microbiome science. This is a fantastic opportunity for an ambitious sales professional to gain hands-on experience and make a tangible impact in a rapidly growing biotech field. Essential Duties and Responsibilities: Sales Support and Development: Assist in the identification and acquisition of new customer accounts, supporting the growth of Sequential’s client base. Client Relationship Building: Cultivate relationships with existing and prospective clients, focusing on understanding their needs and promoting Sequential’s unique offerings. Market Research: Conduct ongoing research to stay informed on industry trends and competitor activity, providing valuable insights for sales strategies. CRM and Data Management: Maintain accurate records in our CRM (HubSpot), tracking interactions, progress, and key performance indicators. Sales Target Achievement: Work towards defined sales goals, contributing to Sequential’s revenue targets and growth ambitions. Collaborative Efforts: Work closely with marketing, product, and operations teams to align efforts and ensure a seamless customer experience. Product Knowledge Development: Stay up-to-date on Sequential’s product offerings and the latest microbiome research to effectively communicate value to clients. Qualifications and Experience: 1-2 years of sales experience, ideally within the biotechnology, cosmetics, or life sciences fields. Familiarity with CRM systems, preferably HubSpot, and the ability to manage data with accuracy. Strong written and verbal communication skills with a passion for client interaction and customer service. Tech-savvy and eager to leverage technology to enhance sales effectiveness. Bachelor's degree in Life Sciences, Business, or a related field preferred; demonstrated interest in microbiome or biotechnology is a plus. Motivated self-starter with a goal-oriented mindset and excellent organizational skills. Ability to work collaboratively and adapt in a dynamic, fast-paced environment. What You Get from Us: A base salary with a commission structure that rewards your hard work and success. Access to our Equity Incentive Plan, allowing you to grow alongside Sequential as we expand. The chance to work with industry experts in one of the fastest-growing sectors within biotechnology. Be part of a company at the forefront of microbiome research, making an impact in the biotechnology and healthcare fields. About the Company Sequential is a global leader in the skin microbiome field, a team of PhD experts in testing products and their effect on the human microbiome (skin, scalp, oral, vulva). An Innovate UK- and Enterprise Singapore-backed company, with labs in London, New York City and Singapore. Awarded the title "Most Significant" Testing Solution in the Industry - (C&T, 2022). To date, Sequential has amassed over 25,000 human skin microbiome samples and corresponding formulations tested in vivo on the skin. With this vast genomic dataset, Sequential are figuring out optimal formulations that could potentially alleviate skin conditions, for example – acne, atopic dermatitis and rosacea. They have been supported by Innovate UK, Enterprise SG, A*STAR (Genome Institute of Singapore). They have raised $4.3MM USD to date from SOSV, Metaplanet, Scrum Ventures, Corundum Systems Biology and are a resident company of JLABS Innovation (in NYC). Apply Now